Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1236282 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2006 | 6 Pages |
Abstract
It is found that nucleic acids can enhance the fluorescence intensity of yttrium(III) (Y3+)-rutin in presence of cetyltrimethylammonium bromide (CTMAB) system. In hexamethylenetetramine (HMTA)-HCl buffer, the maximum enhanced fluorescence is produced, with maximum excitation and emission wavelengths at 452 and 520Â nm, respectively. Based on this, a new fluorimetric method of determination of nucleic acids is proposed. Under optimum conditions, the enhanced fluorescence intensity is proportion to the concentration of nucleic acids in the range of 1.0Â ÃÂ 10â7 to 1.0Â ÃÂ 10â5Â g/ml for fish sperm DNA (fsDNA), 1.0Â ÃÂ 10â7 to 4.6Â ÃÂ 10â6Â g/ml for yeast RNA (yRNA), their detection limits (S/NÂ =Â 3) are 7.5Â ÃÂ 10â8, 8.0Â ÃÂ 10â8Â g/ml, respectively. The interaction mechanism is also studied.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xia Wu, Changying Guo, Fei Wang, Jinghe Yang, Dehuan Ran, Jinhua Zheng, Jinbo Wu,