Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1236413 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2006 | 5 Pages |
Abstract
It is found that in hexamethylene tetramine (HMTA)-HCl buffer of pH 7.00, nucleic acids can quench the resonance light scattering (RLS) of europium (III) (Eu3+)-2-thenoyltrifluoroacetne (TTA)-1,10-phenanthroline (Phen) system. Based on this, a sensitive method for the determination of nucleic acids is proposed. The experiments indicate that under the optimum conditions, the quenched RLS intensity is in proportion to the concentration of nucleic acids in the range of 1.0 Ã 10â10 to 2.0 Ã 10â6 g mlâ1 for fish sperm (fsDNA), 1.0 Ã 10â11 to 1.0 Ã 10â6 g mlâ1 for yeast RNA (yRNA), 5.0 Ã 10â11 to 5.0 Ã 10â7 g mlâ1 for calf thymus DNA (ctDNA). Their detection limits (S/N = 3) are 0.03, 0.006 and 0.002 ng mlâ1, respectively. Therefore, the proposed method is the most sensitive RLS method for the determination of nucleic acids so far. The interaction between nucleic acids and Eu3+-TTA-Phen is also discussed.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Zhen Jia, Jinghe Yang, Xia Wu, Changxia Sun, Shufang Liu, Fei Wang, Zongshan Zhao,