| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1236457 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2011 | 9 Pages |
In this paper, the chlorophyll derivatives, metallochlorophyllin (Chl-M) (M = Fe, Zn and Cu) including chlorophyllin iron (Chl-Fe), chlorophyllin zinc (Chl-Zn) and chlorophyllin copper (Chl-Cu), were adopted as sonosensitizers to combine with ultrasonic irradiation, and the sonodynamic damage of bovine serum albumin (BSA) was investigated. At first, the interaction of Chl-M with BSA was studied by fluorescence spectroscopy. The results show that the quenching mechanism belongs to a static process and among them the affinity of Chl-Fe to BSA is the most obvious. Then, some influence factors on the sonodynamic damage of BSA molecules in the presence of Chl-M under ultrasonic irradiation were also studied. Synchronous fluorescence spectra show that the binding and damage sites of Chl-M to BSA molecule are mainly on the tryptophan (Trp) residues. The generation of ROS in Chl-M sonodynamic process is estimated by the method of Oxidation-Extraction Spectrometry (OEP). This paper may offer some valuable references for the study of the sonodynamic activity of Chl-M and the effect of the central metals. Synchronously, it contributes to the application of Chl-M in SDT for tumor treatment.
Graphical abstractThe metallochlorophyllin (Chl-M) were adopted as sonosensitizers to combine with ultrasonic irradiation, and the sonodynamic damage of bovine serum albumin (BSA) was investigated. The order of the sonodynamic activities is Chl-Fe > Chl-Zn > Chl-Cu. Synchronous fluorescence spectra show that the damage sites of Chl-M to BSA are mainly on the tryptophan (Trp) residues of BSA. The reactive oxygen species (ROS) generated during sonodynamic process was estimated as 1O2 and OH by the method of Oxidation-Extraction Spectrometry (OEP).Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► The interaction of metallochlorophyllin with BSA was studied. ► The sonodynamic damage of BSA in the presence of Chl-M was studied. ► The formations of ROS during sonocatalytic process were studied. ► The kinds of ROS were determined by adding some scavengers.
