Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1236983 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2005 | 5 Pages |
Abstract
A new spectrofluorimetric method was developed for determination of adenosine disodium triphosphate (ATP). We studied the interactions between oxytetracycline (OTC)-Eu3+ complex and adenosine disodium triphosphate (ATP) by using UV-vis absorption and fluorescence spectra. Using oxytetracycline (OTC)-Eu3+ as a fluorescence probe, under the optimum conditions, ATP can remarkably enhance the fluorescence intensity of the OTC-Eu3+ complex at λ = 612 nm and the enhanced fluorescence intensity of Eu3+ ion is in proportion to the concentration of ATP. Optimum conditions for the determination of ATP were also investigated. The linear ranges for ATP are 8.00 Ã 10â8-1.50 Ã 10â6 mol Lâ1 with detection limits of 2.67 Ã 10â9 mol Lâ1. This method is simple, practical and relatively free interference from coexisting substances and can be successfully applied to determination of ATP in samples. The mechanism of fluorescence enhancement between oxytetracycline (OTC)-Eu3+ complex and ATP was also studied.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Faju Hou, Yanhong Miao, Chongqiu Jiang,