Article ID Journal Published Year Pages File Type
1236985 Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2005 6 Pages PDF
Abstract
A highly sensitive spectrofluorimetric method is proposed for determination of human serum albumin (HSA) and some quinolone drugs. Using quinolones-terbium (Tb3+) complex as a fluorescent probe, in the buffer solution of pH 7.8, HSA can remarkably enhance the fluorescence intensity of the quinolones-Tb3+ complex at 545 nm and the enhanced fluorescence intensity of Tb3+ ion is in proportion to the concentration of HSA and quinolone drugs. Optimum conditions for the determination of HSA were also investigated. The linear ranges and limits of detection are 8.0 × 10−9 to 8.0 × 10−8 mol L−1, 4.20 × 10−9 mol L−1 (for HSA); 1.0 × 10−6 to 4.0 × 10−6 mol L−1, 1.87 × 10−8 mol L−1 (for norfloxacin) and 1.0 × 10−7 to 1.0 × 10−6 mol L−1, 4.82 × 10−8 mol L−1 (for enoxacine), respectively. This method is simple, practical and relatively free interference from coexisting substances, as well as much more sensitive than most of the existing assays.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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