Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1236985 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2005 | 6 Pages |
Abstract
A highly sensitive spectrofluorimetric method is proposed for determination of human serum albumin (HSA) and some quinolone drugs. Using quinolones-terbium (Tb3+) complex as a fluorescent probe, in the buffer solution of pH 7.8, HSA can remarkably enhance the fluorescence intensity of the quinolones-Tb3+ complex at 545 nm and the enhanced fluorescence intensity of Tb3+ ion is in proportion to the concentration of HSA and quinolone drugs. Optimum conditions for the determination of HSA were also investigated. The linear ranges and limits of detection are 8.0 Ã 10â9 to 8.0 Ã 10â8 mol Lâ1, 4.20 Ã 10â9 mol Lâ1 (for HSA); 1.0 Ã 10â6 to 4.0 Ã 10â6 mol Lâ1, 1.87 Ã 10â8 mol Lâ1 (for norfloxacin) and 1.0 Ã 10â7 to 1.0 Ã 10â6 mol Lâ1, 4.82 Ã 10â8 mol Lâ1 (for enoxacine), respectively. This method is simple, practical and relatively free interference from coexisting substances, as well as much more sensitive than most of the existing assays.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yusheng Wang, Lin Feng, Chongqiu Jiang,