Article ID Journal Published Year Pages File Type
1237158 Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2005 4 Pages PDF
Abstract

We established a simple and selective fluorimetric method for the determination of ascorbic acid (AA) with the novel mono-[6-N(4-carboxy-phenyl)]-β-cyclodextrin (ACD). The method is based on the fluorescence intensity of ACD decreases as the ACD–AA supramolecular complexes form. The fluorescence intensity is measured at excitation and emission wavelengths of 270 and 352 nm, respectively. Under optimum condition, a linear relationship is obtained between the fluorescence intensity and the concentration of AA in the range of 0.05–8.0 μg ml−1. The detection limit is 0.012 μg ml−1. The method has been applied to the direct analysis of AA in real samples with satisfactory results.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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