Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1238157 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2008 | 6 Pages |
Abstract
The characteristics of host-guest complexation between p-(p-sulfonated benzeneazo) calix[6]arene (SBC6A) and vitamin K3 (VK3) were investigated by fluorescence spectrometry. A 1:1 stoichiometry for the complexation was established and was verified by Job's plot. An association constant of 4.95 Ã 103 L molâ1 at 20 °C was calculated by applying a deduced equation. The interaction mechanism of the inclusion complex was discussed. It was found that the fluorescence of SBC6A could be remarkably quenched by an appropriate amount of VK3 especially when non-ionic surfactant Triton X-100 existed. According to the obtained results, a novel sensitive spectrofluorimetric method for the determination of VK3 based on supramolecular complex was developed with a linear range of 5.0 Ã 10â7-3.0 Ã 10â5 mol Lâ1 and a detection limit of 2.0 Ã 10â7 mol Lâ1. The proposed method was used to determine VK3 in commercial preparations with satisfactory results.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yunyou Zhou, Hongwei Xu, Lian Wu, Chun Liu, Qin Lu, Lun Wang,