Article ID Journal Published Year Pages File Type
1238542 Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2006 6 Pages PDF
Abstract

The Fe3O4/(sodium oleic acid/ethyltrimethyl ammonium bromide)n/4-aminobenzoic acid (Fe3O4/(NaOL/CTAB)n/PABA) nanocomposites have been prepared by a layer-by-layer self-assembly approach. This kind of nanocomposites have fluorescent, magnetic and water-soluble properties. Taking advantage of the magnetic property of nanocomposites, we can separated them from solution easily by using a permanent magnet. By using their strong fluorescence, we can detect proteins. At pH 6.98, the fluorescence of Fe3O4/(NaOL/CTAB)n/PABA nanocomposites can be enhanced by the proteins. Under optimal conditions, the linear ranges of calibration curves were 0.2–20, 0.2–13, 0.2–10 μg mL−1 for γ-globulin (γ-IgG), human serum albumin (HSA), and bovine serum albumin (BSA), respectively. The detection limits were 0.02, 0.01, 0.02 for γ-IgG, HSA and BSA, respectively. The method has been applied to analyze the total proteins in human samples and the results were in good agreement with those reported by the hospital. This method is sensitive, simple and potential in many areas.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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