NMR study on cis-N-[4-[4-(1,2-benzisozole-3-yl)-1-piperazinyl] butyl]cyclohexane-1,2-dicarboximide monohydrochloride dihydrate

Cis-N-[4-[4-(1,2-benzisozole-3-yl)-1-piperazinyl]butyl]cyclohexane-1,2-dicarboximide monohydrochloride dihydrate was studied spectroscopically. Complete NMR assignments were made using DEPT, H–H COSY, as well as HMQC and HMBC heteronuclear correlation techniques. The hydrochloride salt was found at δ > 10. The dihydrate was presence in the region δ 3–4 in DMSO-d6 solvent. Asymmetry carbon C3 brought chemical-shift-nonequivalent of cis-cyclohexanyl group, splitting four systems H1, H1′H1′, H2 and H2′H2′. Diamagnetic anisotropy of benzisozolyl group results in three troops peaks of piperazinyl group.