Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1238899 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2005 | 5 Pages |
Abstract
A direct spectrophotometric determination of DNA in solution has been developed using a near infrared probe, 1,1′-disulfobutyl-3,3,3′,3′-tetramethylindotricarbocyanine (DSTCY). In pH = 7.5 Tris–HCl buffer, the reaction of DSTCY with DNA was complete within 10 min at room temperature in the presence of cetyltrimethylammonium bromide (CTAB), which leaded to a sharp increase of the absorbance at 474 nm. The concentration of DNA can be determined in such wavelength with the linear range of 0.5–8.0 μg ml−1 and the detection limit of 45 ng ml−1.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Hong Wang, Wei-Rong Li, Yin Lu, Ni-Na Fu, Hua-Shan Zhang,