Article ID Journal Published Year Pages File Type
1239103 Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2019 6 Pages PDF
Abstract
Fluorescence-enhancement of 1,2,4-trihydroxyanthroquinone (THAQ)-Be2+ complex enhanced by nucleic acid was studied. Experimental results revealed that double-stranded DNA can enhance remarkably the fluorescence intensity of THAQ-Be2+, while RNA cannot. Based on these results, a fluorescence method for the selective determination of DNA in the presence of RNA was developed. Maximum fluorescence intensity was found in the pH range 3.6-4.5 with maximum excitation and emission wavelengths at 510 and 565 nm, respectively. Under optimum conditions, the calibration graph was linear over the range 0.08-18 μg ml−1 for double-stranded fish sperm DNA (fsDNA) with the detection limit being 2.75×10−8 g ml−1. The method was applied for the determination of DNA in synthetic samples. The relative S.D. for five replicates was within 4%. In addition, the interaction mechanism of THAQ-Be2+ with DNA was also discussed.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
, , , , , ,