Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1241150 | Spectrochimica Acta Part B: Atomic Spectroscopy | 2008 | 5 Pages |
Abstract
The x-ray standing wave method has been applied to study self-assembling processes in a protein-lipid film formed by injecting the protein-lipid mixture of alkaline phosphatase and phosphatidylinositol under the phospholipid monolayer preliminarily deposited on the water subphase by Langmuir method. X-ray standing wave measurements allowed to determine the composition of the protein-lipid film and to locate ions position in the direction normal to the film surface. The presence of trace Ni contamination incorporated in the protein-lipid film from the water subphase has been established. Numerical analysis of the X-ray standing wave fluorescence data revealed that after injection under the phospholipid monolayer, the protein-lipid mixture separated in a self-assembled manner to layered structure, molecules of alkaline phosphatase arranged themselves into a pure protein layer containing no phospholipid molecules.
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Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
S. Zheludeva, N. Novikova, N. Stepina, E. Yurieva, O. Konovalov,