| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1241700 | Talanta | 2016 | 8 Pages |
•A preconcentration method is developed for indirect detection of acrylamide in foods by FAAS.•Ion-pairing, fluorescein in presence of Ni(II) at pH 9.0 is employed for sensitivity improvement.•The method provides a low detection limit enough to meet the food safety requirements.•The method is validated through the analysis of two CRMs.•The method is successfully applied to indirect detection of acrylamide in selected foodstuffs.
Acrylamide is a toxic species for human health, and is a Maillard reaction product which forms spontaneously in heat treatment process of foods. Therefore, a simple, fast and cost-effective method was developed for the indirect determination of acrylamide in processed foods particularly consumed by children. The method is based on ion–pairing of acrylamide with fluorescein (F2−) in presence of Ni(II) ions at pH 9.0, and then extraction of the formed ternary complex into micellar phase of poly(ethyleneglycol-mono-p-nonylphenylether) (PONPE 7.5) before analysis by flame atomic absorption spectrometry (FAAS). The ultrasonic-assisted cloud point extraction (UA-CPE) has been used for the preconcentration of acrylamide in the samples prior to its FAAS detection. The matrix matched calibration curve is linear in range of 0.3–150 µg kg−1 under optimal reagent conditions (1.75 mL of 0.1 mol L−1 ammonia buffer at pH 9.0, 2.2 mg L−1 Ni(II), 4.0×10−4 mol L−1 F2−, 0.4% (w/v) NH4Cl and 0.7% (v/v) PONPE 7.5) with sensitivity enhancement of 160-fold. The proposed method has been validated by assessment of the following parameters; the limits of detection (LOD) and quantification (LOQ) (0.08 µg kg−1 and 0.28 µg kg−1, respectively) with a relative standard deviation (RSD%) lower than 6.3%, and extractive recovery higher than 95% for acrylamide spiked at levels of 5 and 25 µg kg−1. The method was successfully applied to the indirect determination of acrylamide in the processed foods and two CRMs with satisfactory results.
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