| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1241851 | Talanta | 2015 | 7 Pages |
•Phosphodiester stationary phases was synthesized on the silica gel support.•Phosphodiester stationary phases exhibit moderate hydrophobicity.•Residual hydroxyl and phosphate group increase stability at high water concentration.•Synthesized stationary phases separate hydrophobic compounds in RP chromatography.•Phosphodiester stationary phases separates polar compounds in HILIC.
The new type of stationary bonded phases for liquid chromatography with immobilized artificial membrane properties was synthesized. Based on the modification of diol-bonded silica gel the phosphodiester stationary phases were obtained. The structures of synthesized material were confirmed by different physico–chemical techniques such as elemental analysis, infrared spectroscopy (FTIR), 13C CP/MAS NMR and in reversed phase and hydrophilic interaction liquid chromatography, where hydrophobic and polar compounds were sufficiently separated. To present the influence of phosphate group on the retention properties, obtained material were compared with Diol-Ester C12 stationary phase.
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