| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1242022 | Talanta | 2015 | 6 Pages |
•New and rapid immunofiltration assay on nitrocellulose.•Sensitivity in the picomolar range in a 5 min assay.•Diagnostic system for resource-limited settings.
Here we present a new and rapid immunofiltration assay for simultaneous detection of HIV p24 and hepatitis B virus antigens. The assay platform is composed of a 13 mm nitrocellulose filter spotted with capturing bioprobes and inserted in a Swinnex® syringe filter holder. Samples and reagents are flown through the nitrocellulose filter by manual pressure on the syringe. A colorimetric detection allows for naked eye results interpretation. The assay provides sensitivity in the picomolar range in just 5 min, even using low volumes of sample in complex matrix. Probe deposition by spotting allows for flexible combinations of different capturing agents and multiple diagnoses; furthermore, the very simple and inexpensive set-up makes the syringe-based immunoassay on paper microarray a suitable diagnostic system for resource-limited settings.
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