The novel voltammetric method for determination of hesperetin based on a sensitive electrochemical sensor

•A novel voltammetric method for determination of hesperetin was proposed.•The resulting sensor showed a significant voltammetric response to hesperetin.•Some dynamic parameters were calculated and a reasonable reaction mechanism of hesperetin at this sensor was proposed.•This work may be a reference for the pharmacological action of hesperetin in clinical study.

A highly sensitive voltammetric sensor, based on reduced graphene oxide on SWCNTs modified glassy carbon electrode (GCE), was constructed and used for sensitive detection of hesperetin. The electrochemical behavior of hesperetin at this sensor was investigated systematically and a novel voltammetric method for determination of hesperetin was proposed. The redox characters of hesperetin was discussed in detail and a reasonable reaction mechanism was proposed also. As the analytical method, the response currents were linear relationship with the hesperetin concentrations in the range of 5.0×10−8 to 3.0×10−6 mol L−1, with a detection limit of 2.0×10−8 mol L−1 (S/N=3). The method was also applied successfully to detect hesperetin in biological samples and Chinese herbal medicine Flos buddlejae with satisfactory results.

Graphical abstractFigure: A highly sensitive voltammetric sensor, based on reduced graphene oxide on SWCNTs modified glassy carbon electrode (ERGO/SWCNTs/GCE), was constructed and used for sensitive detection of hesperetin. Compared with bare GCE, there is a dramatic increase of the redox peak currents. Then the electrochemical behavior of hesperetin was carefully and systematically investigated and some kinetics parameters were calculated for the first time. The novel electroanalytical method of hesperetin was also established and applied in biological and Chinese herbal medicine samples analysis with satisfactory results.Figure optionsDownload full-size imageDownload as PowerPoint slide