| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1242197 | Talanta | 2016 | 8 Pages |
•The simultaneous detection of ATP, ADP and AMP was established by qNMR.•This method was simple, rapid, sensitive, little sample preparation and green.•It showed excellent linearity in the range of 0.1–100 mM and 1–75 mM, respectively.
ATP, ADP and AMP are energy substances with vital biological significance. Based on the structural differences, a simple, rapid and comprehensive method has been established by 1H and 31P Nuclear Magnetic Resonance (1H-NMR and 31P-NMR) spectroscopies. Sodium 3-(trimethylsilyl) propionate-2,2,3,3-d4 (TMSP) and anhydrous disodium hydrogen phosphate (Na2HPO4) were selected as internal standards for 1H-NMR and 31P-NMR, respectively. Those three compounds and corresponding internal standards can be easily distinguished both by 1H-NMR and 31P-NMR. In addition, they all have perfect linearity in a certain range: 0.1–100 mM for 1H-NMR and 1–75 mM for 31P-NMR. To validate the precision of this method, mixed samples of different concentrations were measured. Recovery experiments were conducted in serum (91–113% by 1H-NMR and 89–113% by 31P-NMR).
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