| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1242334 | Talanta | 2012 | 6 Pages |
We report for the first time an ultra performance liquid chromatographic method with tandem mass spectrometric detection (UPLC/MS/MS) for the determination of norethindrone alone in human plasma over the concentration range of 50.0–25 000 pg mL−1 using a sample volume of 0.250 mL. Norethindrone and its internal standard (ISTD), norethindrone-13C2, were extracted from human plasma by supported liquid extraction (SLE). After evaporation of the organic solvent, samples were reconstituted and analyzed on an UPLC/MS/MS system. The UPLC system used a Waters BEH C18 (100 mm × 2.1 mm, 1.7 μm) column with mobile phase A of 0.05% formic acid in water:acetonitrile (65:35, v/v) and mobile phase B of 0.05% formic acid in methanol:acetonitrile (50:50, v/v). The flow rate was 0.500 mL min−1. The method was fully validated. The inter-run accuracy and precision at the lower limit of quantitation (LLOQ), low, mid and high quality control (QC) concentration levels were 99.2–108.4% with a <8.1% CV (coefficient of variation), respectively. The validated method has been successfully applied to analysis of thousands of pharmacokinetic samples.
► The first published LC/MS/MS method for norethindrone alone in human plasma. ► Simple, fast, convenient and very reproducible extraction. ► Significantly reduced extraction and LC/MS/MS analysis time. ► Fully validated, high precision, accuracy and selectivity were demonstrated. ► Successfully applied to analysis of thousands of pharmacokinetic samples.
