Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1242396 | Talanta | 2011 | 6 Pages |
Abstract
A thermo-responsive and macroporous monolithic cryogel was directly prepared by in situ free-radical redox cryo-polymerization in a stainless steel chromatographic column (100 mm Ã 4.6 mm i.d.) using N-isopropylacrylamide (NIPAAm) as functional monomer and PEG-20,000 as porogen at â12 °C. The internal morphology of resulting monolithic cryogel was estimated by scanning electron microscopy (SEM). Based on that, a submicron skeleton structure was observed. Besides, the gravimetrically determined rates of swelling/deswelling for thermo-responsive monolithic cryogel were much higher than that of hydrogel adopting the same component proportion via conventional method (25 °C for 24 h). Simultaneously, a temperature-dependent resolution of steroids was also achieved using only water as a mobile phase. The theoretical plate number of every analyte was more than 2000.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Haiyan Liu, Mingquan Liu, Ligai Bai, Suying Sun, Yankun Liu, Gengliang Yang,