| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1242587 | Talanta | 2016 | 10 Pages |
•We have developed Ti4+-immobilized capillary trapping column (250 μm i.d.) for on-line enrichment of phosphopeptides.•It minimizes the sample loss and improves the effectiveness of phosphopeptide detection that the limit of detection is as low as 1 fmol/μL.•It is successfully applied to the detection of phosphopeptides fromskim milk, human serum and mouse brain.
In this work, we have developed Ti4+-immobilized capillary trapping column (250 μm i.d.) for highly specific on-line enrichment of phosphopeptides in the bio-samples. It minimizes the sample loss and improves the effectiveness of phosphopeptide detection that the limit of detection is as low as 1 fmol/μL. It is successfully applied to the detection of phosphopeptides from complex biological samples, such as skim milk, human serum and mouse brain. The results indicate that the Ti4+-immobilized capillary trapping column is time-effective and has the great potential of application in low-abundance phosphopeptides on-line analysis. The prepared Ti4+-immobilized capillary trapping column will be further used in LC/MS platform for phosphoproteome analysis.
Graphical abstractHerein, we present Ti4+-immobilized capillary trapping column (250 μm i.d.) for highly specific on-line enrichment of phosphopeptides in the bio-samples.Figure optionsDownload full-size imageDownload as PowerPoint slide
