Article ID Journal Published Year Pages File Type
1242678 Talanta 2010 6 Pages PDF
Abstract

A rapid, low-cost, high sensitive and quantitative method to detect valiolamine in a medium for microbial culture, involving derivatization with a new labeling reagent, 4-methoxybenzenesulfonyl fluoride (MOBS-F), followed by reverse-phase high-performance liquid chromatography with ultraviolet (UV) detection with simple operation procedure. 4-Methoxybenzenesulfonyl chloride (MOBS-Cl) and 2-nitrobenzenesulfonyl chloride (NBS-Cl) were compared with MOBS-F as novel reagents in this paper, and the MOBS-F was chosen as the most suitable derivatization reagent. The column was thermostatic at 35 °C, the mobile phase flow-rate was 1.0 mL/min and the detection wavelength was 240 nm. For a biological sample, the separation of the derivatives was achieved using a gradient mobile system. The elution program is 88% phosphate buffer (50 mM; pH = 3.0) and 12% methanol for 23 min, then 70% of phosphate buffer and 30% methanol for another 15 min and finally 88% of phosphate buffer and 12% of methanol for 5 min to re-equilibrate the column. The optimized conditions of the derivatization were as follows: derivatization reaction temperature 30 °C; derivatization reaction pH value 11.0, reaction time 10 min and MOBS-F concentration higher than 1.5 mg/mL for standard solutions and higher than 5.0 mg/mL for the biological sample. Calibration curves were linear in the range of 0.050–25 μg/mL for the standard solutions and 1.0–75 μg/mL for the biological sample. The sensitive analytical method is helpful to control the biotechnological process of voglibose production and product quality control.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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