| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1242782 | Talanta | 2016 | 5 Pages |
•A NBD-based reversible fluorescent probe was synthesized.•Probe displayed selective On–Off–On fluorescent responses to ClO−/AA redox cycle.•Fluorescent bioimaging of ClO−/AA in HeLa cells was enabled.
Based on the reversible redox reaction of 4-amino-2,2,6,6-tetramethyl-piperidin-1-ol with oxidative ClO− and reductive ascorbic acid (AA), a reversible fluorescent probe, incorporating 4-amino-2,2,6,6-tetramethyl-piperidin-1-ol group into a 7-nitrobenzoxadiazole (NBD) fluorophore, was synthesized and studied for the cyclic detection of ClO−/AA in aqueous solution. The NBD-based probe displayed sensitive and selective On–Off–On fluorescent responses to ClO−/AA and was successfully applied to monitor the ClO−/AA redox cycle in living HeLa cells under physiological conditions.
Graphical abstractA reversible fluorescence probe has been developed and was successfully applied to monitor the ClO−/AA redox cycle in both aqueous solutions and living HeLa cells.Figure optionsDownload full-size imageDownload as PowerPoint slide
