Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1242835 | Talanta | 2010 | 5 Pages |
Abstract
A novel electrochemical DNA sensor was developed here by using peroxidase-like G-quadruplex-based DNAzyme as a biocatalytic label. A hairpin structure including the G-quadruplex-based DNAzyme in a caged configuration and the target DNA probe were immobilized on Au-electrode surface. Upon hybridization with the target, the hairpin structure was opened, and the G-quadruplex-based DNAzyme was generated on the electrode surface, triggering the electrochemical oxidization of hydroquinone by H2O2, which provide a quantitative measure for the detection of the target DNA. The DNA target was analyzed with a detection limit of 0.6 nM. This method is simple and easy to design without direct conjugation of redox-active element.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Qiuping Guo, Yanyan Bao, Xiaohai Yang, Kemin Wang, Qing Wang, Yuyu Tan,