| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1242896 | Talanta | 2013 | 5 Pages |
A new method using reversed phase capillary liquid chromatography was developed for simultaneous determination of four bioactive compounds found in ginger (Zingiber officinale) namely, 6-, 8-, 10-gingerol, and 6-shogaol. The separation of these four compounds was performed using C30 as the stationary phase and 60% acetonitrile as the mobile phase in isocratic elution mode with a flow rate of 5 μL/min. All four compounds were separated within 25 min with good resolution. As the evaluation of method validation, a linear regression of the four compounds was obtained within the tested range with correlation coefficients ≥0.9995. The limits of detection and quantitation were between 0.034–0.039 μg/mL and 0.112–0.129 μg/mL, respectively. Intra- and inter-day precision expressed as relative standard deviations (RSD) were less than 3.1%, and the accuracy based on recovery test was ranging from 97% to 105%. Stability of the analytes within 1 day was found in the range between 1.34% and 2.93% (RSD). In addition, based on the amount of these four compounds combining with the discriminant analysis, a reliable and accurate method was developed for discrimination of three ginger varieties found in Indonesia. The results indicated that the developed method could be used as quality control for ginger raw material and its related products.
► Determination of gingerols and shogaol in ginger by capillary LC was conducted. ► Discriminant analysis could distinguish the three ginger varieties. ► Capillary LC and discriminant analysis could be used for quality control of ginger.
