Article ID Journal Published Year Pages File Type
1242975 Talanta 2010 7 Pages PDF
Abstract

A new electrochemiluminescence (ECL) DNA assay is developed using quantum dots (QDs) as DNA labels. When nanoporous gold leaf (NPGL) electrodes are used, sensitivity of the ECL assay is remarkably increased due to ultra-thin nanopores. In this assay, target DNA (t-DNA) is hybridized with capture DNA (c-DNA) bound on the NPGL electrode, which is fabricated by conjugating amino-modified c-DNA to thioglycolic acid (TGA) modified at the activated NPGL electrode. Following that, amino-modified probe DNA is hybridized with the t-DNA, yielding sandwich hybrids on the NPGL electrode. Then, mercaptopropionic acid-capped CdTe QDs are labeled to the amino group end of the sandwich hybrids. Finally, in the presence of S2O82− as coreactant, ECL emission of the QD-labeled DNA hybrids on the NPGL electrode is measured by scanning the potential from 0 to −2 V to record the curve of ECL intensity versus potential. The maximum ECL intensity (Im,ECL) on the curve is proportional to t-DNA concentration with a linear range of 5 × 10−15 to 1 × 10−11 mol/L. The ECL DNA assay can be used to determine DNA corresponding to mRNA in cell extracts in this study.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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