| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1243269 | Talanta | 2016 | 7 Pages |
•A virus-MIPs fluorescent assay for the detection of virus was reported firstly.•The target could be captured specifically by JEV-MIPs via the recognition cavities.•A FRET enhancement strategy for the specific recognition of virus was obtained.•Highly sensitive detection of JEV in water was offered with wide linear range.
Major stumbling blocks in the recognition and detection of virus are the unstable biological recognition element or the complex detection means. Here a fluorescent sensor based on virus-molecular imprinted polymers (virus-MIPs) was designed for specific recognition and highly sensitive detection of Japanese encephalitis virus (JEV). The virus-MIPs were anchored on the surface of silica microspheres modified by fluorescent dye, pyrene-1-carboxaldehyde (PC). The fluorescence intensity of PC can be enhanced by the principle of fluorescence resonance energy transfer (FRET), where virus acted as energy donor and PC acted as energy acceptor. The enhanced fluorescence intensity was proportional to the concentration of virus in the range of 24–960 pM, with a limit of detection (LOD, 3σ) of 9.6 pM, and the relative standard deviation was 1.99%. In additional, the specificity study confirmed the resultant MIPs has high-selectivity for JEV. This sensor would become a new key for the detection of virus because of its high sensitive, simple operation, high stability and low cost.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide
