Article ID Journal Published Year Pages File Type
1243477 Talanta 2010 5 Pages PDF
Abstract

To our knowledge, we report the first fluorescence aptasensor for detecting human neutrophil elastase (HNE) in homogeneous solution. The biosensor contains a short DNA scrambled sequence strand (SS) complementary to part of the aptamer sequence or the loop of molecular beacon (MB). The aptamer-HNE recognition event involves competition between the molecular beacon and loose HNE aptamer for the binding the short DNA strand. The new biosensor can detect as little as 0.34 nM of HNE, and the response is linear in the tested concentration range of 0.34–68 nM with the detection limit of 47 pM.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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