Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1243486 | Talanta | 2010 | 8 Pages |
Abstract
We developed a method to graft a tripeptide (glutathione) onto 5-hydroxy-1,4-naphthoquinone, an electropolymerizable molecule. The resulting thin conducting polymer presents a well-defined and stable electroactivity in neutral buffered solution, due to the embedded quinone group, and is able to covalently graft amino-modified DNA probe strands. It is shown that the bioelectrode presents positive current change following DNA hybridization. This makes a “signal-on” direct electrochemical DNA sensor. The results were obtained with low target concentration (50 nM) and the selectivity is excellent as a single-mismatch sequence can be discriminated from the full-complementary target.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
S. Reisberg, D.F. Acevedo, A. Korovitch, B. Piro, V. Noel, I. Buchet, L.D. Tran, C.A. Barbero, M.C. Pham,