Article ID Journal Published Year Pages File Type
1243529 Talanta 2008 5 Pages PDF
Abstract

A pentafluorophenylpropyl (PFPP) stationary phase was for the first time tested for the simultaneous determination of triamcinolone acetonide, its degradation product triamcinolone and two preservatives, methylparaben, and propylparaben. A new simple isocratic reversed phase HPLC method with UV detection, using estradiol hemihydrate as an internal standard, has been developed and validated. Chromatography was performed on a Discovery HS F5 column (150 mm × 4.6 mm, 5 μm) using a binary mobile phase composed of acetonitrile and water 45:55 (v:v). The flow-rate was 0.6 mL/min, the column temperature 25 °C and the UV detection was accomplished at 240 nm.The chromatography results using PFPP stationary phase were compared with those obtained using conventional C18 columns.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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