| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1243585 | Talanta | 2014 | 8 Pages |
•CUPRAC and Fe-Phen methods were modified to be used in untreated edible oils.•The total reducing power of different kinds of edible oils was estimated.•The results were compared to those of the DPPH and Folin–Ciocalteu methods.•The methods are simple and fast so can be used for routine oil analysis.
The lipophilicity of untreated edible oils narrows the application of most published methods for the determination of antioxidant activity to hydrophilic extracts of oils.This research addresses the issue of the estimation of the total antioxidant properties of untreated edible oils by modifying two widely applied analytical methods, the Fe-Phenanthroline and the CUPRAC assays, to be used in untreated oils. The modifications pertain to the selection of mixture of solvents (ethanol–butanol in 3:1 v/v ratio), and the optimization of the reaction conditions (reagents concentration and reaction time).The developed methods were applied to a number of hydrophilic and lipophilic standard compounds and different types of commercial edible oils, as well as their corresponding aqueous or organic extracts. This implementation elucidated the differences in the antioxidant content of edible oils. All the results were compared to those of the DPPH and Folin–Ciocalteu methods and the analytical figures of merit for the methods have been estimated.Lastly, it was concluded that the modified CUPRAC assay has higher sensitivity compared to the Fe-Phenanthroline assay.
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