| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1243618 | Talanta | 2014 | 5 Pages |
•Simultaneous derivatization of glycols and glycolic acid with phenylboronic acid and chloroformate in serum/urine.•Sample preparation time within 10 min suitable for emergency toxicology.•Optimization of derivatization conditions with experimental statistical design.•Successfull quantitave analysis of glycols and glycolic acid in real human serum samples of poisoned persons.
A simple, cost effective, and fast gas chromatography method with flame ionization detection (GC-FID) for simultaneous measurement of ethylene glycol, 1,2-propylene glycol and glycolic acid was developed and validated for clinical toxicology purposes. This new method employs a relatively less used class of derivatization agents – alkyl chloroformates, allowing the efficient and rapid derivatization of carboxylic acids within seconds while glycols are simultaneously derivatized by phenylboronic acid. The entire sample preparation procedure is completed within 10 min. To avoid possible interference from naturally occurring endogenous acids and quantitation errors 3-(4-chlorophenyl) propionic acid was chosen as an internal standard. The significant parameters of the derivatization have been found using chemometric procedures and these parameters were optimized using the face-centered central composite design. The calibration dependence of the method was proved to be quadratic in the range of 50–5000 mg mL−1, with adequate accuracy (92.4–108.7%) and precision (9.4%). The method was successfully applied to quantify the selected compounds in serum of patients from emergency units.
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