Bianalyte multicommutated flow analysis system for microproteinuria diagnostics

•Flow analysis system useful for microproteinuria diagnostics is presented.•The system is based on optoelectronics and microsolenoid devices.•This flow analysis system is useful for estimation of protein creatinine ratio index.

In this work a bianalyte multicommutated flow analysis (MCFA) system for determination of microproteinuria is presented. The developed MCFA system is based on two dedicated optoelectronic flow-through detectors which allow estimation of urinary protein creatinine ratio. For total protein determination, turbidimetric Exton's method was used, whereas creatinine was determined by the photometric Jaffe reaction. The developed analytical system is fully-mechanized, easy to operate, economic in reagent consumption and characterized by satisfactory analytical parameters. It allows protein determination in the range 36–300 mg L−1 with 33 mg L−1 detection limit and simultaneous determination of creatinine in the range 0.045–2.50 mmol L−1 with 0.025 mmol L−1 detection limit. The measurement procedure for the presented MCFA system offers performing 30 peaks per hour for both analytes. To prove the analytical usefulness of the system, real human urine samples have been analyzed. The correlation and agreement between results offered by the developed system and clinical analyzers are fully acceptable.

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