Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1243909 | Talanta | 2008 | 7 Pages |
Abstract
Direct determination of serotonin (5-HT) in gut lavage fluid from patients examined due to various gastrointestinal complaints has been achieved. The method involves addition of 5-methoxytryptamine (5-CH3O-HT) internal standard, centrifugation, filtration and injection of the sample supernatant in a liquid chromatographic system coupled to an ion trap tandem mass detector. Electro-spray in positive mode was used to isolate and fragment the protonated ions [5-HTÂ +Â H]+ and [5-CH3O-HTÂ +H]+ signals 177 and 191Â m/z, respectively. Quantification was carried out by extracting the ion fragment chromatograms at 160 and 174Â m/z for 5-HT and 5-CH3O-HT, respectively. The relationship 5-HT/5-CH3O-HT was modelled by using a simultaneous design in order to estimate the optimal amount of internal standard to be added to the samples prior to quantification.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Kine Gregersen, Livar Frøyland, Arnold Berstad, Pedro Araujo,