Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1243979 | Talanta | 2008 | 6 Pages |
Abstract
A novel flow injection (FI)-spectrofluorimetric methodology for the determination of carvedilol in microheterogeneous medium has been developed. In the sodium dodecyl sulfate (SDS) surfactant medium, an additional fluorescence enhancement was produced by the electrolyte NaCl. A total enhancement of 3.1-fold in the native fluorescent response was achieved respect to aqueous medium. Using an excitation and emission wavelength of 286 and 341 nm, respectively, a good linear relationship was obtained in the range of 9 Ã 10â8 to 1 Ã 10â6 mol Lâ1 with a detection limit of 3.63 Ã 10â9 mol Lâ1 (S/N = 3). This method was applied to determine carvedilol in commercial pharmaceutical formulations. Good concordance was found between the nominal (6.25, 12.5 and 25.0 mg) and experimental values. The new methodology developed showed high selectivity respect to the common excipients used in pharmaceuticals. The sampling rate was 30 samples hâ1. From the fluorescent properties, binding constant for carvedilol-SDS determined was 3.2 Ã 102 L molâ1.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Raúl A. Silva, Chien Chun Wang, Liliana P. Fernández, Adriana N. Masi,