| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1244068 | Talanta | 2015 | 6 Pages |
•Developed a method to use a glucose meter to make discrete amperometric measurements for ethanol detection on a micro-paper electrochemical device.•Results were comparable to those obtained with a potentiostat.•Inclusion of trehalose was critical for effective measurements after 24 hours of device preparation.
Recent advances in electrochemical analysis on filter paper exemplify the versatility of this substrate for high performance testing. Its low-cost, light-weight, and environmentally friendly properties make it particularly attractive for applications in addressing health and environmental safety needs in low-resource settings and developing countries. However, the main drawback to sensitive electrochemical testing is the use of a potentiostat, a bench-top instrument that is extremely expensive, thereby negating the some of the benefits of paper-based devices. Hence there is a need to develop paper-devices for use with handheld, portable device readers that can extract quantitative readouts. In this study, we developed a method to use micro-paper electrochemical devices, or µPEDs, with a glucose meter, which are used for personal monitoring of blood glucose levels. Ethanol was chosen as a model target analyte due to its importance in the global issue of road safety. µPEDs were simple in design and could be tested with a potentiostat. We observed that inclusion of the stabilizer trehalose was critical to preparing µPEDs for later analysis. In addition, an NAD+-dependent enzyme was used to impart selectivity to the biosensor, which also represents a class of enzymes with targets relevant to the health and food industry.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide
