Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1244218 | Talanta | 2007 | 5 Pages |
Abstract
Direct electrochemical and electrocatalytic behavior of hemoglobin (Hb) immobilized on glass carbon electrode (GCE) containing gelatine (Gel) films was investigated. The characteristics of Hb/Gel film modified GC electrode were performed by using SEM microscopy, UV-vis spectroscopy and electrochemical methods. The immobilized Hb showed a couple of quasi-reversible redox peak with a formal potential of â0.38Â V (versus SCE) in 0.1Â M pH 7.0 PBS. The formal potential changed linearly from pH 4.03 to 8.41 with a slope value of â52.0Â mV pHâ1, which suggested that a proton transfer was accompanied with each electron transfer (ET) in the electrochemical reaction. The Hb/gelatine/GCE displayed a rapid amperometric response to the reduction of H2O2 and nitrite.
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Authors
Hui Yao, Nan Li, Jin-Zhong Xu, Jun-Jie Zhu,