Article ID Journal Published Year Pages File Type
1244377 Talanta 2010 5 Pages PDF
Abstract

A method using automated on-line solid-phase extraction (SPE) liquid chromatography–electrospray tandem mass spectrometry (LC–ESI-MS/MS) for the determination of 8-iso-PGF2α in human exhaled breath condensate (EBC) was developed and validated. A C18 SPE column with an affinity sorbent was used for on-line extraction. A C18 column was employed for LC separation and ESI-MS/MS was utilized for detection. 8-iso-PGF2α-d4 was used as an internal standard for quantitative determination. The extraction, cleanup and analysis procedures were controlled by a fully automated six-port switch valve. Identification and quantification were based on the following transitions: m/z 353 → 193 for 8-iso-PGF2α and m/z 357 → 197 for 8-iso-PGF2α-d4, respectively. Good recoveries from 98.94 to 99.86% were measured and satisfactory linear ranges for these analytical compounds were determined. Intra-day and inter-day precision showed that coefficients of variance (CV) ranged from 6.5 to 8.0% and 5.2 to 6.3%, respectively. The applicability of this newly developed method was demonstrated by analyzing human EBC samples for an evaluation of the future risk of human exposure to nanoparticles.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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