Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1244524 | Talanta | 2008 | 7 Pages |
Abstract
Gold nanoparticles (AuNPs) were synthesized in situ and electrodeposited onto Au substrate. The AuNPs modified interface facilitates electron transfer across self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid (MUA). After activation of surface carboxyl groups with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide, the interface displayed good stability for immobilization of biomolecules. These modification processes were characterized by contact angle measurement, cyclic voltammetry and electrochemical impedance spectra. The immobilized acetylcholinesterase (AChE), as a model, showed excellent activity to its substrate, leading to a stable AChE biosensor. Under the optimal experimental conditions, the inhibition of malathion on AChE biosensor was proportional to its concentration in two ranges, from 0.001 to 0.1 μg mLâ1 and from 0.1 to 25 μg mLâ1, with detection limit of 0.001 μg mLâ1. The simple method showed good reproducibility and acceptable stability, which had potential application in biosensor design.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Dan Du, Jiawang Ding, Jie Cai, Jianming Zhang, Li Liu,