Investigation on enhanced chemiluminescence reaction systems with bis(hydrogenperiodato) argentate(III) complex anion for fluoroquinolones synthetic antibiotics

A novel chemiluminescence (CL) reaction system with bis(hydrogenperiodato) argentate(III) complex anion (Ag(III) complex, [Ag(HIO6)2]5−), for the first time, is developed for the determination of lomefloxacin (LMFX), enrofloxacin (ENLX) and pefloxacin (PFLX). The possible CL emission mechanism was discussed by comparing the fluorescence emission with CL spectra. The CL conditions of [Ag(HIO6)2]5−–H2SO4–LMFX/ENLX/PFLX systems were investigated and optimized. Under the optimized experimental conditions, the CL intensity is proportional to the concentration of the drugs in the range 0.2994–36.80 × 10−7 g mL−1 for LMFX, 4.00–30.0 × 10−7 g mL−1 for ENLX and 1.54–27.64 × 10−7 g mL−1 for PFLX. The limit of detection (s/n = 3) was 9.1 × 10−9 g mL−1 for LMFX, 3.1 × 10−9 g mL−1 for ENLX and 4.4 × 10−9 g mL−1 for PFLX. The recovery of LMFX, ENLX and PELX from the spiked pharmaceutical preparations was in the range of 92.3–105% with the RSDs of 0.5–2.7%. For urine, serum and milk samples the recoveries of the three drugs were in the range of 85.1–107% for LMFX with the RSDs of 2.3–3.4%. 80.2–112% for ENLX with the RSDs of 1.4–2.8%, and 87.8–114% for PFLX with the RSDs of 1.6–2.7%. The proposed method was applied successfully to the determination of these compounds in real samples.