Simultaneous chemiluminescence determination of promazine and fluphenazine using support vector regression

In this work, chemiluminescence (CL) behaviors of two selected phenothiazines, namely promazine and fluphenazine hydrochloride, were investigated for their simultaneous determination using oxidation of Ru(bipy)32+ by Ce4+ ions in acidic media. This method is based on the kinetic distinction of the CL reactions of fluphenazine and promazine with Ru(bipy)32+ and Ce4+ system in a sulfuric acid medium. Least square support vector regression models were constructed for relating concentrations of both compounds to their CL profiles. The parameters of the model consisting of σ2 and γ were optimized using all possible combinations of σ2 and γ to select the model with the minimum root mean square cross validation. Under optimized conditions, the univariate calibration curve was linear over the concentration ranges of 0.4–30.0 μg mL−1 and 0.07–5.0 μg mL−1 with detection limits of 0.1 μg mL−1 and 0.04 μg mL−1 for promazine and fluphenazine, respectively. The influence of potential interfering substances on the determination of promazine and fluphenazine were studied. The proposed method was used for simultaneous determination of both compounds in synthetic mixtures and in spiked human plasma.