Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1244916 | Talanta | 2009 | 6 Pages |
An ion mobility spectrometer equipped with an ultraviolet lamp was used for the qualitative and quantitative determination of acetone in urine samples. This analyte can be used as a biomarker for some fat metabolism-related diseases in humans and cows. Samples require no pretreatment other than warming at 80 °C for 5 min, after which an N2 stream is used to drive volatile analytes to the ion mobility spectrometer. The precision of the ensuing method, expressed as relative standard deviation (%RSD), is better in all cases than 6.7% for peak height and calculated at three levels of concentration. The analyte concentration range studied was from 5 to 80 mg L−1, its limit of detection in the aqueous matrix 3 mg L−1 and recoveries from spiked urine samples 109 ± 3%. The calculated reduced mobility for acetone in the urine samples, 1.75 ± 0.04 cm2 V−1 s−1, was similar to previously reported values. Also, the results were consistent with those provided by test strips used for reference. The proposed method provides a new vanguard screening system for determining acetone in urine samples.