Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1244931 | Talanta | 2009 | 5 Pages |
Abstract
A capillary zone electrophoresis method was developed for simultaneous determination of nine flavonoids, including two rare flavonols, in Tibetan medicine Anaphalis margaritacea. Baseline separation was performed at pH 9.6 with 25 mM Na2B4O7 and 10 mM NaH2PO4 buffer solution, 20 kV as driving voltage and 275 nm as detection wavelength. Repeatability tests showed that the R.S.D. of both intra- and inter-day migration times and peak areas were less than 5%. Recovery results ranged from 87.9% to 106.1%. Samples of A. margaritacea extracts were analyzed using the validated method, which is useful for its quality control.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Zhao-Yan Ren, Yu Zhang, Yan-Ping Shi,