Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1244991 | Talanta | 2007 | 4 Pages |
Abstract
A new simple and sensitive analytical spectrophotometric method is developed for the determination of ascorbic acid reduces methyl viologen to form a stable blue coloured free radical ion. This method has a sensitivity and lower limit detection of 0.1 μg ml−1 of ascorbic acid (0.1 ppm) which is comparable to the flow injection analysis reported earlier. Beer's law is obeyed over the concentration range of 1.0–10 μg ml−1 of ascorbic acid per 10 ml of the final solution (0.1–1.0 μg ml−1) at 600 nm. The molar absorptivity and Sandell's sensitivity were found to be 1.5 × 105 ± 100 l mol−1 cm−1 and 0.001 μg cm−2, respectively. The method has been applied to the determination of ascorbic acid in food, pharmaceuticals and biological samples.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
E.K. Janghel, V.K. Gupta, M.K. Rai, J.K. Rai,