Article ID Journal Published Year Pages File Type
1245107 Talanta 2009 6 Pages PDF
Abstract

A sensitive amperometric sensor for determination of l-histidine was developed using gold electrode modified with Fe(III)-porphyrin bearing three 2,6-di-tert-butylphenol groups and one palmitoyl chain. Two methods of electrode modification were applied: direct chemisorption and embedment into dodecanethiol monolayer. Both types of electrodes were used for detection of l-histidine using Osteryoung square-wave voltammetry. The sensitivity of sensors presented towards l-histidine depends on the method of electrode modification. The detection limits observed for the electrodes incorporating with Fe(III)-porphyrin host by embedment and chemisorption were in 1 and 100 nM ranges, respectively. In addition, the determination of l-histidine with electrode modified by embedment technique was more precise, in comparison to that obtained by the direct chemisorption.Applicability of gold electrodes modified with Fe(III)-porphyrin for the direct electrochemical determination of l-histidine was demonstrated using the artificial matrix mimicking human serum.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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