| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1245157 | Talanta | 2007 | 4 Pages |
Abstract
A reverse flow injection spectrophotometric enzymatic method is proposed to quantify total phenols in urine samples. The polyphenol oxidase (PPO; EC 1.14.18.1) obtained as a crude extract from sweet potato root (Ipomoea batatas) was used as enzymatic catalyze. The detection limit, the sample throughput and relative standard deviation were 7.7 mg l−1 of total phenols, 49 h−1 and 0.9%, respectively. The method was applied to real samples and a recovery study was carried out in order to its validation.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Marcos Grünhut, Miriam E. Palomeque, Adriana G. Lista, Beatriz S. Fernández Band,