Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1245212 | Talanta | 2009 | 6 Pages |
Abstract
Fatty acids of forty-two samples of perirenal fat of goat kids reared on three different feeding systems: goat milk (B), milk replacer (R) and milk-based starter (F) have been analyzed by Gas Chromatography flame ionization detector. The lipids were extracted by melting of perirenal fat in a microwave oven. The fat was then filtered and dissolved in hexane. This analysis was performed on a column (100 m Ã 0.25 mm i.d. and 0.25 μm film thickness) coated with a polar stationary phase HP-88 and flame ionization detector was used. Hydrogen (25 psi inlet constant pressure) was used as carrier gas. Programmed temperature was kept at 175 °C and held isothermally for 10 min, and was then raised to 205 °C at a rate of 3 °C/min and held isothermally for 10 min. By using the fatty acids as chemical descriptors, pattern recognition techniques were applied to differentiate between goat milk, milk replacer and milk-based starter fattening diet of goat kid. C18:2 and C18:3 acids were found to be the most differentiating variables.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Tania Mellado-González, Mónica Narváez-Rivas, M. Jesús Alcalde, Tomás Cano, Manuel León-Camacho,