Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1245216 | Talanta | 2009 | 5 Pages |
Abstract
This work shows the possibility of combining the high sensitivity of genetically-modified Drosophila melanogaster acetylcholinesterase (B394) with the ability of phosphotriesterase (PTE) to hydrolyse organophosphate compounds, in the aim of developing a biosensor selective to two insecticides of interest: chlorpyrifos and chlorfenvinfos. The studies clearly demonstrate that chlorfenvinfos is a substrate that acts as competitive inhibitor of PTE, therefore preventing the efficient hydrolysis of other pesticides, including chlorpyrifos. A bi-enzymatic sensor was designed by immobilizing both B394 and PTE in a polyvinylalcohol matrix. The sensor was shown to be able to discriminate between chlorpyrifos and chlorfenvinfos inhibitions.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Georges Istamboulie, Didier Fournier, Jean-Louis Marty, Thierry Noguer,