Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1245407 | Talanta | 2008 | 6 Pages |
Abstract
In this paper, the long-chained, silanizing agent chloro(dodecyl)dimethylsilane (CDDS) was investigated as a semipermanent coating in micellar electrokinetic capillary chromatography (MEKC). CDDS coating had great stability due to the formation of covalent bonding with the silanol groups on the surface of fused-silica capillary and remained stable for over 100 min after removal of the rinse step of CDDS solution. Anionic surfactant sodium dodecyl sulfate (SDS) could aggregate at this CDDS coating by the hydrophobic group and formed a SDS layer which could increase the electroosmotic flow (EOF). The separation was performed with the running buffer composed of 60 mM sodium tetraborate, 12 mM SDS at pH 9.9, with the applied voltage of 20 kV and capillary temperature 25 °C. The effect of the coating agent was investigated by the analysis of amino acids. Compared with previous no-coating method, the EOF increases from 4.34 Ã 10â4 cm2 Vâ1 sâ1 to 7.02 Ã 10â4 cm2 Vâ1 sâ1. Migration time reproducibility was less than 0.97% R.S.D. from run to run and less than 1.56% R.S.D. from day to day.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Zhi Luo, Shumin Wang, Lei Zhou, Zhide Hu,