Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1245414 | Talanta | 2008 | 4 Pages |
Abstract
Traditional methods to assay enzymatic cleavage of DNA are discontinuous, time-consuming and laborious. Here, we report a new approach for real-time monitoring of double-stranded DNA cleavage by restriction endonuclease based on nucleic acid ligation using molecular beacon. Upon cleavage of DNA, the cleavage product can be ligated by DNA ligase, which results in a fluorescence enhancement of the molecular beacon. This method permits real-time monitoring of DNA cleavage and makes it easy to characterize the activity of restriction endonuclease and to study the cleavage reaction kinetics.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Changbei Ma, Zhiwen Tang, Xiqin Huo, Xiaohai Yang, Wei Li, Weihong Tan,