Article ID Journal Published Year Pages File Type
1245452 Talanta 2008 6 Pages PDF
Abstract

Molecularly imprinted polymers using serotonin as the template molecule was prepared for selective recognition from platelet rich plasma by non-covalent imprinting approach. Four different monomers (methacrylic acid, acrylamide, 4-vinylpyridine and 2-acrylamido-2-methylpropane sulfonic acid) and acetonitrile and DMSO as porogen were investigated for the first time by bulk polymerization. The molecularly imprinted polymer which was prepared by acrylamide/methacrylic acid had the largest imprinting factor for serotonin. The affinity and specificity of these polymers were evaluated by equilibrium binding experiments. The effect of polarity of the solvents was examined by polymers binding capacity and imprinting factor. According to the Scatchard analysis the Kd and Qmax values were calculated as 1.95 μmol l−1 and 19.129 μmol g−1, respectively. The polymer was tested to evaluate serotonin from platelet rich plasma and 70% serotonin recovery was found.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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